Molecular Physiology

Guest Research Group

We analyze ciliary signaling and ciliary function in health and disease.

Publications

Balbach, M., Beckert, V., Hansen, J.N. & Wachten, D. (2018) “Shedding light on the role of cAMP in mammalian sperm physiology” Mol. Cell. Endocrinol. 468, 111-120

Woeste, M. & Wachten, D. (2017) “The enigmatic role of GBA2 in controlling locomotor function” Front. Mol. Neurosci. 10, 386

Von Gerichten, J., Schlosser, K., Lamprecht, D., Morace, I., Eckhardt, M., Wachten, D., Jennemann, R., Gröne, M., Macke, M. & Sandhoff, R. (2017) “Diastereomer-specific quantification of bioactive hexosylceramides from bacteria and mammals” J. Lipid Res. 58, 1247–1258

Körschgen, H., Kuske, M., Kramilin, K., Yiallouros, I., Balbach, M., Floehr, J., Wachten, D., Jahnen-Dechent, W. & Stöcker, W. (2017) “Intracellular activation of ovastacin mediates pre-fertilization hardening of the zona pellucida ” Mol. Hum. Reprod. 23, 607-616

Jansen, V., Jikeli, J.F., Wachten, D. (2017) "How to control cyclic nucleotide signaling by light" Curr. Opin. Biotechnol. 48, 15 - 20

Schonauer, S., Körschen, H.G., Penno, A., Rennhack, A., Breiden, B., Sandhoff, K., Gutbrod, K., Dörmann, P., Raju, D.N., Haberkant, P., Gerl, M.J., Brügger, B., Zigdon, H., Vardi, A., Futerman, A.H., Wachten, D. (2017) "Identification of a feedback loop involving beta-glucosidase 2 and its product sphingosine sheds light on the molecular mechanisms in Gaucher disease" J. Biol. Chem., M116.762831

Berger, T.K., Fußhöller, D.M., Goodwin, N., Bönigk, W., Müller, A., Khesroshahi, N.D., Brenker, C., Wachten, D., Krause, E., Kaupp, U.B. & Strünker, T. (2017) "Posttranslational cleavage of Hv1 in human sperm tunes pH- and voltage-dependent gating” J. Physiol. 595, 1533-1546

Schneider, S., Balbach, M., Jikeli, J., Fietz, D., Nettersheim, D., Jostes, S., Schmidt, R., Kressin, M., Bergmann, M., Wachten, D., Steger, K. & Schorle, H. (2016) "Re-visiting the Protamine-2 locus: deletion, but not haploinsufficiency, renders male mice infertile" Sci. Rep. 6, 36764

Wachten, D., Jikeli, J. & Kaupp, U.B. (2016) "Sperm sensory signaling" CSH Perspect. Biol., a028225

Björkgren, I., Alvarez, L., Blank, N., Balbach, M., Turunen, H., Laajala, T.D., Toivanen, J., Krutskikh, A., Wahlberg, N., Huhtaniemi, I., Poutanen, M., Wachten, D. &  Sipilä, P. (2016) "Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding" Mol. Cell. Endocrinol. 427, 143-154

Mukherjee, S., Jansen, V., Jikeli, J.F., Hamzeh, H., Alvarez, L., Dombrowski, M., Balbach, M., Strünker, T., Seifert, R., Kaupp, U.B. & Wachten, D. (2016) "A novel biosensor to study cAMP dynamics in cilia and flagella" eLife 5, e14052

Raju, D., Schonauer, S., Hamzeh, H., Flynn, K.C., Bradke, F., vom Dorp, K., Dörmann, P., Yildiz, Y., Trötschel, C., Poetsch, A., Breiden, B., Sandhoff, K., Körschen, H.G. &  Wachten, D. (2015) "Accumulation of glucosylceramide in the absence of the Beta-Glucosidase GBA2 alters cytoskeletal dynamics" PLoS Genet 11, e1005063

Jansen, V., Alvarez, L., Balbach, M., Strünker, T., Hegemann, P., Kaupp, U.B. & Wachten, D. (2015) "Controlling fertilization and cAMP signaling in sperm by optogenetics" eLife 4, e05161  

Schiffer, C., Müller, A., Egeberg, D.L., Alvarez, L., Brenker, C., Rehfeld, A., Frederiksen, H., Wäschle, B., Kaupp, U.B., Balbach, M., Wachten, D., Skakkebaek, N.E., Almstrup, K. & Strünker, T. (2014) "Direct action of endocrine disrupting chemicals on human sperm" EMBO Rep.15, 731-815

Mass, E.*, Wachten, D.*, Aschenbrenner, A.C., Voelzmann, A. & Hoch, M. (2014) "Murine Creld1 Controls Cardiac Development through Activation of Calcineurin/NFATc1 Signaling" Dev. Cell 28, 711-726
*equally contributed

Krähling, M., Alvarez, L., Debowski, K., Van, Q., Gunkel, M., Irsen, S., Al-Amoudi, A., Strünker, T., Kremmer, E., Krause, E., Voigt, I., Wörtge, S., Waisman, A., Weyand, I., Seifert, R., Kaupp, U.B. & Wachten, D. (2013) "CRIS- a novel cAMP-binding protein controlling spermiogenesis and the development of flagellar bending" PLoS Genetics, DOI: 10.1371/journal.pgen.1003960

Rabionet, M., Bayerle, A., Marsching, C., Jennemann, R., Grone, H.J., Yildiz, Y., Wachten, D., Shaw, W., Shayman, J.A. & Sandhoff, R. (2013) "1-O-Acylceramides are natural components of human and mouse epidermis" J. Lipid. Res. 54,3312-3321

Körschen, H.G., Yildiz, Y., Raju, D.N., Schonauer, S., Bönigk, W., Jansen, V., Kremmer, E., Kaupp, U.B. & Wachten, D. (2013) "The non-lysosomal beta-glucosidase GBA2 is a non-integral membrane-associated protein at the ER and Golgi" J. Biol. Chem. 288, 3388-3393

 

Drawnel, F.M.*, Wachten, D.*, Molkentin, J.D., Maillet, M., Aronsen, J.M., Swift, F., Sjaastad, I., Liu, N., Catalucci, D., Mikoshiba, K., Hisatsune, C., Okkenhaug, H., Andrews, S.R., Bootman, M. D. & Roderick, H.L. (2012) "Mutual antagonism between IP3RII and miRNA-133a regulates calcium signals and cardiac hypertrophy" J. Cell. Biol. 199, 783-798
*equally contributed

Harzheim, D., Roderick, H.L. & Bootman, M.D. (2011) "Intracellular calcium signaling" In: Bradshaw, R.A. & Dennis, E.A. (eds.): Functioning of transmembrane receptors in cell signaling (Amsterdam: Academic Press), 377-382

Smyrnias, I., Mair, W., Harzheim, D., Walker, S.A., Roderick, H.L. & Bootman, M.D. (2010) "Comparison of the T-tubule system in adult rat ventricular and atrial myocytes, and its role in excitation-contraction coupling and inotropic stimulation" Cell Calcium 47, 210-223

Harzheim, D., Talasila, A., Movassagh, M., Foo, R.S., Figg, N., Bootman, M.D. & Roderick, H.L. (2010) "Elevated InsP(3)R expression underlies enhanced calcium fluxes and spontaneous extra-systolic calcium release events in hypertrophic cardiac myocytes" Channels (Austin) 4, 67-71

Harzheim, D., Movassagh, M., Foo, R.S., Ritter, O., Tashfeen, A., Conway, S.J., Bootman, M.D. & Roderick, H.L. (2009) "Increased InsP3Rs in the junctional sarcoplasmic reticulum augment Ca2+ transients and arrhythmias associated with cardiac hypertrophy" Proc. Natl. Acad. Sci. USA 106, 11406-11411

Harzheim, D., Seifert, R., Pfeiffer, K.H., Fabritz, L., Kremmer, E., Buch, T., Waisman, A., Kirchhof, P. & Kaupp, U.B. (2008) "Cardiac pacemaker function of HCN4 channels is confined to embryonic development and requires cyclic AMP" EMBO J. 27, 692-703

Roderick, H.L., Higazi, D.R., Smyrnias, I., Fearnley, C., Harzheim, D. & Bootman, M.D. (2007) "Calcium in the heart: when it's good, it's very very good, but when it's bad, it's horrid" Biochem. Soc. Trans. 35, 957-961

Bootman, M.D., Harzheim, D., Smyrnias, I., Conway, S.J. & Roderick, H.L. (2007) "Temporal changes in atrial EC-coupling during prolonged stimulation with endothelin-1" Cell Calcium 42, 489-501