Diffraction limited imaging in biological tissue is limited due to aberrations and scattering. These limitations of optical microscopy drive the development of adaptive optics techniques.
In this publication, Ivan Vishniakou and Johannes Seelig describe a method for wavefront correction in adaptive optics with reflected light and deep neural networks.
Vishniakou, I. and Seelig, J.D. (2020). Wavefront correction for adaptive optics with reflected light and deep neural networks Opt Express28, 15459-15471.